The designation of a Natura 2000 site ROSCI0434 Siretul Mijlociu for the protection of habitats and species, whose development is closely tied to the aquatic environment, signifies that the management of water resources, and implicitly, the water footprint, has contributed to maintaining a balanced and favorable status for biodiversity conservation. Given that the Natura 2000 site was declared based on older studies, and the Management Plan for the protected natural area was not yet developed, we are now conducting an assessment of the impact of the water footprint in the current climatic conditions. This includes considering other predominant types of agricultural crops and changes in food habits. This evaluation aims to understand the relationship between the water footprint and the conservation status of species and habitats in light of these new circumstances. The blue and grey water components of the water footprint are the ones that directly affect the natural protected area out of the three components of the water footprint: green, blue, and grey water. The ecological impact of water use in ROSCI0434 Siretul Mijlociu is extensive, affecting both terrestrial and aquatic ecosystems. Pollutants can harm habitats and species, while agricultural water usage alters habitats and disrupts hydrological patterns, impacting breeding and foraging grounds. Additionally, changes in water quantity and quality diminish ecosystem services like water purification and habitat provision, affecting biodiversity and human well-being. Display omitted •Water footprint gauge human impact on water supplies and ecosystems.•Includes consumption, population growth, water infrastructure in water footprint.•Grey water important for habitat viability and species conservation.