Purpose The ability to actively adjust walking speed is fundamental and the factors enabling it should be assessed. The present study aimed to demonstrate how active gait speed is kinematically adjusted. Participants and Methods Walking acceleration and deceleration were evaluated in 16 healthy adults using three-axis accelerometers and surface electromyographs. The root mean square (RMS) of each axis in the center-of-gravity acceleration was calculated as an index of gait stability. Electron myograph data were obtained from images captured of the right lower muscles, and the integral value of total muscle activity per gait cycle was calculated. Results The RMS of each axis increased during acceleration and decreased during deceleration. The integral values of total activity of the gastrocnemius, biceps femoris, and tibialis anterior muscles increased in acceleration. In contrast, the values increased in the biceps femoris but decreased in other muscles during deceleration. Conclusion These results suggest that the specific kinematic mechanisms of each factor regulate the acceleration and deceleration of walking. In addition, these mechanisms and factors indicate how exercise therapy may be used in rehabilitation to improve the ability to adjust walking speed in daily life.