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Zhao, Ning-ning; Liu, Wen-jing; Tian, Xiaorui; Zhang, Baogang; Zhang, Chun-yang
Chemical communications (Cambridge, England), 01/2023, Letnik: 59, Številka: 8Journal Article
We demonstrate that target-activated cascade transcription amplification lights up RNA aptamers for label-free detection of metalloproteinase-2 (MMP-2) activity with zero background. This assay exhibits good specificity and high sensitivity with a limit of detection (LOD) of 0.6 fM. Moreover, it can analyze enzyme kinetic parameters, screen inhibitors, and accurately quantify MMP-2 in cancer cells and clinical serums. Target-activated cascade transcription amplification lights up RNA aptamers for label-free detection of metalloproteinase-2 activity with zero background.
