The recent development and commercialization of microelectromechanical systems (MEMS)/nanoelectromechanical systems (NEMS) has brought the related challenge of independently powering such systems. The concept of the nanogenerator (NG) has shown potential for harvesting energy from the ambient environment to power MEMS/NEMS. Kinetic energy harvesting NGs based on the piezoelectric properties of ZnO nanowires have attracted much interest. In this paper, we have fabricated hydrothermally synthesized ZnO-based NGs following the procedures standardized in the published literature. Likewise, reference NGs without ZnO piezoelectric material were fabricated in parallel with the ZnO NGs. The voltage output of both the ZnO NG and the reference NG was measured given a 10-Hz cyclic vertical load. Unexpectedly, both the ZnO and the reference NG were found to produce 3 mV with 0.451 N of load. A finite-element model was created to determine that the voltage potential of the NG should be about 1 mV with the given load. A possible explanation for this unexpected behavior is that the measured signals are not entirely piezoelectric in nature. Rather, the signals recorded from the NGs may be some alternate phenomenon, such as the triboelectric, flexoelectric, or electret effect.