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Recenzirano
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Abbruzzese, L.; Agostini, F.; Durante, C.; Toffola, R. T.; Rupolo, M.; Rossi, F. M.; Lleshi, A.; Zanolin, S.; Michieli, M.; Mazzucato, M.
Vox sanguinis, July 2013, Letnik: 105, Številka: 1Journal Article
Peripheral blood stem cell cryopreservation is associated with cell damage and decreased viability. We evaluated the impact of up to 10 years of cryopreservation (5% DMSO) on viability of CD34+ cells utilizing graft samples of consecutive patients (2002–2012) with different malignancies who underwent stem cell collection and transplantation. Viability of CD34+ cells from oncohaematological patients measured after 5 weeks (97·2 ± 0·6%) or after 9–10 years of cryopreservation (95·9 ± 0·5%) was unaffected. Haemoglobin, granulocyte and platelet recovery after transplantation of long‐term cryopreserved grafts occurred within 8–13 days. CD34+ stem cells can be safely stored up to 9–10 years, without affecting cell viability and clinical effectiveness.
