Myxobacteria can produce rich and varied biological active substances against bacteria, fungi and viruses, which have great development and research value in medicine and agriculture. In this study, the diversity of culturable myxobacteria in central Inner Mongolia in China was studied and the effects of soil utilization mode, soil type and environmental parameters on the distribution of myxobacteria in this region were analyzed. Furthermore, the activities of myxobacteria against potato late blight pathogen were tested. The results showed that Myxococcus, Corallococcus, Pyxidicoccus, Cystobacter, Archangium and Mellittangium were the dominant genera of myxobacteria in this region. Soil utilization mode and soil types have obvious influence on the distribution of myxobacteria. The populations of myxobacteria were abundant in grassland and cultivated land samples, but few in woodland and unused land samples. The diversity of myxobacteria in the soil samples from fuvo-aquic soils, grey-cinnamon soils, castanozems, and bog soils was relatively rich, while the richness of myxobacteria in aeolian soils, solonetzs, skeletol soils and castano-cinnamon soils was poor. There was no significant correlation between myxobacteria distribution and soil environmental parameters (including the water content, pH value, content of organic matter, content of available phosphorus, content of hydrolytic nitrogen and content of available potassium). Most of the myxobacterial strains isolated in this area (83%) showed the activity against P. infestans, among which the proportion of the disease-resistant strains belonging to Myxococcus and Corallococcus was high, the proportion of the strains belonging to Cystobacter and Mellittangium in the medium, and the proportion of the strains belonging to Pyxidicoccus and Archangium low. The completion of this work will enrich the myxobacteria resource bank in Inner Mongolia and lay a foundation for the further study on myxobacteria and the development of biological pesticide against potato late blight. © 2021 Friends Science Publishers