The aim of this research was to investigate genetic specificity of sunflower nutrition with microelements. Therefore, concentration of essential (Zn, B, Mn, Cu, Fe, and Ni) and non-essential (Cr, Al, Cd, As, Pb, and Ba) micronutrients were analyzed. Five sunflower hydrids the most grown in Serbia and different populations of wild sunflower species originating from North America: Helianthus neglectus, Helianthus agrophyllus, Helianthus petiolaris, Helianthis annuus were included in the experiment. Populations of wild sunflower species and hybrids differed significantly with respect to the concentration of analyzed elements. Coeficient variation of microelement concentration depended on genotype and particular elements. In wild populations, for essential microelements, it was between 3.7 and 59.5, whereas in hybrids it varied from 10.0 to 48.8. Coefficient variation of concentration of non-essential microelements in wild populations varied from 7.7 to 73.8, and in hybrids from 15.1 to 48.8. Average coefficient of variation in both wild species and hybrids was the lowest for Mn and Pb. It was the highest for Cr, Ni, and Zn in hybrids and for Cr, Ni, and Cr in wild species. The results suggest that genetic specificity with respect to uptake of microelements in wild species and hybrids is highly expressed.