E-viri
Recenzirano
Odprti dostop
-
Shortall, Kim; Arshi, Simin; Bendl, Simon; Xiao, Xinxin; Belochapkine, Serguei; Demurtas, Denise; Soulimane, Tewfik; Magner, Edmond
Green chemistry : an international journal and green chemistry resource : GC, 06/2023, Letnik: 25, Številka: 11Journal Article
The use of enzymes in biochemical processes is of interest due to their ability to work under mild conditions while attaining high reaction rates. A limitation in the use of enzymes such as oxidoreductases on a large scale lies with their requirement for costly cofactors, e.g. NAD + , in stoichiometric quantities. Cofactor regeneration mechanisms using bienzymatic recycling systems is an attractive way to increase productivity and efficiency. The thermophilic enzyme aldehyde dehydrogenase (ALDH Tt ) was immobilized directly from E. coli cell lysate, containing the expressed enzyme, onto Ni 2+ activated Sepharose®. The system displayed a rate of conversion of approx. 63% NAD + with reuse achievable for up to 5 cycles and residual activity of the enzyme upon storage of 93% after 7 days. l -Lactate dehydrogenase was immobilized in a second reactor module downstream of ALDH Tt via two different methods, electrochemical entrapment in poly(3,4-ethylenedioxypyrrole) (PEDOP) and covalent attachment on glyoxyl agarose. Both reactors allowed for up to 100% conversion of NADH, however LDH@agarose proved superior in terms of reuse and storage. LDH@agarose displayed no reduction in activity after 6 cycles of use and retained 98% activity following 56 days storage. A coupled reactor containing immobilized ALDH Tt -LDH was operated with the substrates hexanal, benzaldehyde, terephthalaldehyde and p -tolualdehyde. A particular advantage of the system is its ability to preferentially oxidise a single aldehyde group in substrates containing two aldehyde functional groups. The reactor demonstrated efficient cofactor regeneration under continual operation for up 24 h, with enhanced product yields. An immobilized bi-enzymatic (ALDH Tt -LDH) flow reactor has been develeoped for the selective oxidation of aldehydes.
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.