With the booming requirements for diabetes management, food quality control, and bioprocess inspection, monitoring of glucose in various matrices has drawn unprecedented interest of both academic and industrial researchers recently. As a relatively new class of glucose sensors, enzyme-free detection of the target is capable of providing several fascinating characters such as ultra-high sensitivity, excellent stability, and simple fabrication. Considering the rapid expansion of the glucose determination field without using any biological enzymes, here we focus our attention on updating the latest advances in non-enzymatic electrochemical glucose sensors based on non-noble transition metal materials achieved in the past few years. In this minireview, both the superiorities and the intrinsic drawbacks of detecting glucose by employing non-precious materials including Ni, Cu, Co, Mn, and Fe are intensively highlighted, followed by a systematic discussion on the important progress harvested for enzymeless glucose sensing. Finally, the potential opportunities of non-noble transition metal materials in fabricating high-performance enzyme-free glucose sensors are given, and the current challenges for their practical applications are also summarized. We summarize the latest advances of non-enzymatic glucose detection using non-noble transition metal materials, highlighting their opportunities and challenges.