Observationally locating the position of the H2O snowline in protoplanetary disks is crucial for understanding planetesimal and planet formation processes, and the origin of water on the Earth. In our studies, we conducted calculations of chemical reactions and water line profiles in protoplanetary disks, and identified that ortho/para-H216O, H218O lines with small Einstein A coefficients and relatively high upper state energies are dominated by emission from the hot midplane region inside the H2O snowline. Therefore, through analyzing their line profiles the position of the H2O snowline can be located. Moreover, because the number density of the H218O is much smaller than that of H216O, the H218O lines can trace deeper into the disk and thus they are potentially better probes of the exact position of the H2O snowline in disk midplane.