Background: Tomato is commonly treated as “Protective Food” as it contain high amounts of several nutritive compounds especially carotenoids such as lycopene and â carotene (Provitamin A), flavonoids, phenolic acids and ascorbic acid and minerals like calcium, iron and phosphorus. Development of high yielding varieties, genetic improvement and best selection are the basic outcome of a superior breeding programme. Therefore, a study was conducted out to analyze the combining ability for yield and yield contributing traits of tomato genotypes. Methods: This study analyzed the general combing ability (GCA) and specific combing ability (SCA) of tomato (Lycopersicon esculantum Mill.) including different breeding lines and landraces, which were grown during three seasons (March 2020, September 2020 and March 2021) with row to row and plant to plant spacing of 60 × 45 cm in randomized block designs (RBD) having three replications at the Agriculture research farm of Choudhary Chhotu Ram (PG) College, Muzaffarnagar U.P., India. Morpho-metric data were recorded on five competitive randomly selected plants in each line for sixteen traits. Result: Number of fruit per plant and fruits yield was governed by non-additive gene action suggesting the advancement of the generation for selection to improve these traits. The parents floradade, arka meghali and EC-164563 were estimated to be best combiners for the inclusion in further breeding programs. The cross EC-620406 × EC-528360, a product of good combiners was best performing and can be used to obtain superior recombinants.