Biosphere Reserves are considered as means for the people who live and work within them to attain a balanced relationship with the natural and semi-natural environment. Moreover, they contribute to the needs of society by showing a way to a more sustainable future. The Wienerwald Biosphere Reserve partly surrounds the city of Vienna and other minor settlements, representing a well-developed example of Green Infrastructure (GI) of great cultural and natural value. Its heterogeneous landscape offers a variety of landscape services (LS). In this work we quantified and mapped the capacity of LS offered by the open land elements of Wienerwald. Starting from a high-resolution dataset, we selected suitable indicator classes, and scored each ecological and socio-cultural service through an expert-based capacity matrix. The subsequent GIS analyses focused on the intensity and density of LS capacities by developing an index useful for mapping GI functionality. The work provides an effective monitoring tool for the Reserve’s both ecological and socio-cultural sustainability performance. It also allows detecting resilient areas, by considering both the spatial distribution and the abundance of landscape elements.