The dataset contains the records of vascular plant species occurrences and distribution in old cemeteries (OC) of the Lower Dnipro region (Southern Ukraine). The analysed cemeteries were located in different types of landscapes (agricultural, rural and urban) and represent various ways of using their area (currently used, closed, abandoned). The floristic list includes 440 species of vascular plants (437 , 3 . The dataset demonstrates a sozological (Red-lists species) value of old cemeteries in Southern Ukraine. The cemeteries constitute refuges of native, rare and steppe flora and play a role of steppe habitat islands in a landscape almost completely transformed to arable land or urbanised. This is the first dataset which contains information about flora of old cemeteries in Lower Dnipro region (Southern Ukraine). The dataset comprises 2118 occurrences of vascular plants (440 species) recorded in the years 2008-2021 in 13 old cemeteries of the Lower Dnipro region. The dataset includes information about 85 occurrences of rare species (23 species , 3 ) and 652 occurrences of 117 steppe species.