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Yoshii, Noriyo; Ogura, Yoshitoshi; Hayashi, Tetsuya; Ajiro, Takashi; Sameshima, Toshiya; Nakazawa, Muneo; Kusumoto, Masahiro; Iwata, Taketoshi; Akiba, Masato
Applied and Environmental Microbiology, 09/2009, Letnik: 75, Številka: 17Journal Article
A total of 905 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates that were recovered from experimentally infected cattle, in addition to the inoculated strain, were analyzed by pulsed-field gel electrophoresis (PFGE). Twelve PFGE profiles other than that of the inoculated strain were observed. We successfully identified five distinct chromosomal deletions that affected the PFGE profiles using whole-genome PCR scanning and DNA sequencing analysis. The changes in PFGE profiles of EHEC O157:H7 isolates after passage through the intestinal tract of cattle were partially generated by deletion of chromosomal regions.
