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Lee, Sangkyu; Park, Hyerim; Kyung, Taeyoon; Kim, Na Yeon; Kim, Sungsoo; Kim, Jihoon; Heo, Won Do
Nature methods, 06/2014, Letnik: 11, Številka: 6Journal Article
We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP.
