The Department of Urology at the Sestre milosrdnice University Hospital Center is the oldest urological institution in the Republic of Croatia and this part of Europe. Today, the Department is a modern tertiary healthcare institution, where the most complex methods of urological practice are performed using modern medical devices and highly sophisticated technology. In 2011, our urology specialist education program was certified by the European Board of Urology (EBU) as the only one of its kind in Croatia. The program was recertified in 2017. The Department runs a program for the early detection of prostate cancer and performs more than 240 radical prostatectomies annually, which is the highest number of such interventions in Croatia. The aim of this study is to present the work and the activities of the Reference Center for Prostate Tumors of the Ministry of Health at the Department of Urology in Sestre milosrdnice University Hospital Center over the last 20 years. The database of the Reference Center for Prostate Tumors of the Ministry of Health at the Department of Urology in Sestre milosrdnice University Hospital Center was reviewed. During the twenty-year period, approximately 15,000 prostate interventions were performed due to benign and malignant diseases. Of this, 7,374 transrectal ultrasound guided prostate biopsies, 2,632 radical prostatectomies with open retropubic access, 3,988 transurethral prostate resections and 1,097 open suprapubic adenomectomies were performed. With the achieved scientific and professional results in monitoring, studying and improving the prevention, diagnosis and therapy of prostate tumors, as well as with the professional conditions and personnel, the Department of Urology in Sestre milosrdnice University Hospital Center truly justifies the title of the Reference Center for Prostate Tumors of the Ministry of Health of the Republic of Croatia awarded to it in 1998.Key words: Prostate; Prostate cancer; Department of Urology; Reference Center for Prostate Tumors; European Board of Urology (EBU)Klinika za urologiju Klinickog bolnickog centra Sestre milosrdnice najstarija je uroloska ustanova u Republici Hrvatskoj, a i u ovom dijelu Europe. Danas je Klinika za urologiju moderna medicinska ustanova tercijarne zdravstvene zastite gdje se najslozenije metode uroloske prakse izvode pomocu suvremenih medicinskih uredaja i visoko sofisticirane tehnologije. Program edukacije specijalizanata je 2011. godine certificiran od strane European Board of Urology (EBU) kao jedini takav u Hrvatskoj, a recertificiran je 2017. godine. U nasoj se Klinici provodi program ranog otkrivanja raka prostate, a godisnje se obavlja preko 240 radikalnih prostatektomija sto je ujedno i najveci broj takvih zahvata u Hrvatskoj. Cilj rada je prikazati rad i aktivnost Referentnog centra Ministarstva zdravstva za tumore prostate u Klinici za urologiju KBC "Sestre milosrdnice" u posljednjih 20 godina. Ucinjen je pregled baze podataka Referentnog centra za tumore prostate Ministarstva zdravstva u Klinici za urologiju KBC "Sestre milosrdnice". Tijekom dvadesetogodisnjeg razdoblja ucinjeno je oko 15 000 zahvata na prostati zbog benignih i malignih bolesti. Od toga je ucinjena 7 374 biopsija prostate vodene transrektalnim ultrazvukom, 2 632 radikalnih prostatekomija otvorenim retropubicnim pristupom, 3 988 transuretralnih resekcija prostate i 1 097 otvorenih suprapubicnih adenomektomija. Postignutim znanstvenim i strucnim rezultatima u pracenju, proucavanju i unaprjedenju prevencije, dijagnostike i terapije tumora prostate kao i strucno-kadrovskim uvjetima, Klinika za urologiju KBC Sestre milosrdnice opravdava naslov Referentnog centra za tumore prostate Ministarstva zdravstva Republike Hrvatske koji nosi od 1998. godine.Kljucne rijeci: Prostata; Karcinom prostate; Klinika za urologiju; Referentni centar za tumore prostate; European Board of Urology (EBU)