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Tarhonskaya, Hanna; Szöllössi, Andrea; Leung, Ivanhoe K. H; Bush, Jacob T; Henry, Luc; Chowdhury, Rasheduzzaman; Iqbal, Aman; Claridge, Timothy D. W; Schofield, Christopher J; Flashman, Emily
Biochemistry (Easton), 04/2014, Letnik: 53, Številka: 15Journal Article
Deacetoxycephalosporin C synthase (DAOCS) catalyzes the oxidative ring expansion of penicillin N (penN) to give deacetoxycephalosporin C (DAOC), which is the committed step in the biosynthesis of the clinically important cephalosporin antibiotics. DAOCS belongs to the family of non-heme iron(II) and 2-oxoglutarate (2OG) dependent oxygenases, which have substantially conserved active sites and are proposed to employ a consensus mechanism proceeding via formation of an enzyme·Fe(II)·2OG·substrate ternary complex. Previously reported kinetic and crystallographic studies led to the proposal of an unusual “ping-pong” mechanism for DAOCS, which was significantly different from other members of the 2OG oxygenase superfamily. Here we report pre-steady-state kinetics and binding studies employing mass spectrometry and NMR on the DAOCS-catalyzed penN ring expansion that demonstrate the viability of ternary complex formation in DAOCS catalysis, arguing for the generality of the proposed consensus mechanism for 2OG oxygenases.
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