E-viri
Recenzirano
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Mehrabi, Pedram; Schulz, Eike C; Agthe, Michael; Horrell, Sam; Bourenkov, Gleb; von Stetten, David; Leimkohl, Jan-Philipp; Schikora, Hendrik; Schneider, Thomas R; Pearson, Arwen R; Tellkamp, Friedjof; Miller, R J Dwayne
Nature methods, 10/2019, Letnik: 16, Številka: 10Journal Article
We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.
