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Senko, Michael W; Remes, Philip M; Canterbury, Jesse D; Mathur, Raman; Song, Qingyu; Eliuk, Shannon M; Mullen, Chris; Earley, Lee; Hardman, Mark; Blethrow, Justin D; Bui, Huy; Specht, August; Lange, Oliver; Denisov, Eduard; Makarov, Alexander; Horning, Stevan; Zabrouskov, Vlad
Analytical chemistry (Washington), 12/2013, Letnik: 85, Številka: 24Journal Article
Proteome coverage and peptide identification rates have historically advanced in line with improvements to the detection limits and acquisition rate of the mass spectrometer. For a linear ion trap/Orbitrap hybrid, the acquisition rate has been limited primarily by the duration of the ion accumulation and analysis steps. It is shown here that the spectral acquisition rate can be significantly improved through extensive parallelization of the acquisition process using a novel mass spectrometer incorporating quadrupole, Orbitrap, and linear trap analyzers. Further, these improvements to the acquisition rate continue to enhance proteome coverage and general experimental throughput.
