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Jacquemart, R; Chavane, N; Durocher, Y; Hoemann, C; De Crescenzo, G; Jolicoeur, M
Biotechnology and bioengineering, 1 May 2008, Letnik: 100, Številka: 1Journal Article
An innovative and automated method for the at-line monitoring of secreted protein was developed by harnessing a Surface Plasmon Resonance-based biosensor to a bioreactor. The proof of concept was performed by following at-line the relative concentration of a secreted protein produced by transient transfection of mammalian cells in a bioreactor. Our results suggest that our approach can be readily applied to the at-line determination of both protein concentration and bioactivity. Our experimental setup and strategy can thus satisfy the needs related to the development of novel bioprocess control protocols in the context of the new process analytical technology that arises in the biopharmaceutical industry. Biotechnol. Bioeng. 2008;100: 184-188.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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