Abstract Routine surveying of academic centers focused on the human-animal bond is critical to understand the trajectory of the field and to create an environment where centers can learn from one another and build collaborations. The purpose of this manuscript was to report the findings of a survey of these human-animal bond centers, to summarize the status of the field, and to identify changes within the field since 2016. Survey questions concentrated on the demographic characteristics, engagement programs, educational opportunities, and research focuses of the centers. Findings suggest that the field continues to grow as one-third of human-animal bond centers are less than 10 years old. The number of centers that participated in this survey increased by 31% compared with the previous survey. Centers have developed a variety of engagement programs, including animal-assisted intervention and companion animal education programs. About half of the centers (48%) offer degree programs and about one quarter of the centers (24%) offer certificate programs. Most centers (95%) focus their research on companion animals with the most studied companion animal being dogs (95%). The most frequent data collection method was surveys (86%). Qualitative analyses, behavior measures, and physiological measures were also common. The most notable changes from the 2016 survey include overall growth of the field (indicated by the establishment of new centers) and a shift in the specialty area of directors, moving from 44% of directors being veterinarians in 2016 survey to 90% having a human-focused specialty in the 2021 survey. Most centers’ research focused on animal-assisted interventions that are consistent with the previous survey. As the field of the human-animal bond continues to grow and more centers emerge, ongoing evaluation of offerings is important to track changes, identify needs, and foster success.