Mature seeds of two traditional rice genotypes (Kalijira and Chinigura) were used for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators cultured on MS (Murashige and Skoog) basal medium. Callus induction frequency was different between the cultivars, as well as among the 2,4- dichlorophenoxyacetic acid (2,4-D) levels tested. Both tested cultivars exhibited highest callus frequency at 2 mgl 2,4-D. The incorporation of benzylaminopurine (BAP) and kinetin (KIN) in the callus induction medium supplemented with 2 mgl 2,4-D did not significantly improve the callus induction frequency but required days of callus initiation were decreased compared to single use of 2,4-D. After two subcultures, at 21 days interval, embryogenic callus was placed on medium containing different concentration and combination of auxin and cytokinin. Treatment T4 (0.5 mg l-1 BAP and 0.1 mgl IBA) showed the highest shoot induction: 91.67% in Kalijira and 83.33% in Chinigura. Similarly, the highest range of shoot number was also observed in both genotypes when treated with 0.5 mgl BAP and 0.1 mgl IBA. Plant regeneration efficiency was further observed best when treated with 1 mgl 2,4-D along with 1 mgl 2,4-D along with 1 mgl BAP and 1 mgl IBA. Furthermore, the highest number of callus derived shoot per culture was achieved in 2 mgl 2,4-D along with 1 mgl BAP and 1 mgl IBA. Both rice genotypes are promising in terms of callus induction frequency and morphology, and regeneration ability of the embryogenic callus. Zrela semena dveh tradicionalnih genotipov riža (‘Kalijira’ and ‘Chinigura’) so bila uporabljena za indukcijo kalusa in regeneracijo rastlin pri različnih koncentracijah in kombinacijah rastlinskih rastnih regulatorjev pri gojenju na osnovnem MS (Murashige and Skoog) mediju. Frekvenca indukcije kalusa je bila različna med sortama kot tudi glede na koncentracije 2,4-diklorfenoksi ocetne kisline (2,4-D). Obe preiskušeni sorti sta imeli največjo frekvenco kalusa pri 2 mgl 2,4-D. Dodatek benzilaminopurina (BAP) in kinetina (KIN) v medij za indukcijo kalusa z dodatkom 2 mgl 2,4-D ni značilno izboljšal indukcije kalusa, vendar so se potrebni dnevi za začetek tvorbe kalusa zmanjšali v primerjavi s postopkom, ko smo uporabili samo 2,4-D. Po dveh predkulturah, v interval 21 dni, je bil embriogeni kalus prenešen na medij, ki je vseboval različno koncentracijo in kombinacijo auksina in citokinina. Tretma T4 (0.5 mg l-1 BAP in 0.1 mgl IBA) je dal največjo indukcijo poganjkov: 91.67 % pri ‘Kalijira’ in 83.33 % pri ‘Chinigura’. Podobno je nastalo največ poganjkov pri obeh sortah, kadar so jih tretirali z 0.5 mgl BAP in 0.1 mgl IBA. Nadalje je bila sposobnost regeneracije rastlin najboljša, če so jih tretirali z 1 mgl 2,4-D z dodatkom 1 mgl BAP in 1 mgl IBA. Največje število iz kalusa nastalih poganjkov na kulturo je bilo doseženo pri 2 mgl 2,4-D z dodatkom 1 mgl BAP in 1 mgl IBA. Oba genotipa riža sta obetavna v smislu morfologije in pogostosti indukcije kalusa kot tudi v regeneracijski sposobnosti embriogenega kalusa.