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Horie, Takahiro; Baba, Osamu; Kuwabara, Yasuhide; Chujo, Yoshimasa; Watanabe, Shin; Kinoshita, Minako; Horiguchi, Masahito; Nakamura, Tomoyuki; Chonabayashi, Kazuhisa; Hishizawa, Masakatsu; Hasegawa, Koji; Kume, Noriaki; Yokode, Masayuki; Kita, Toru; Kimura, Takeshi; Ono, Koh
Journal of the American Heart Association, December 2012, Letnik: 1, Številka: 6Journal Article
Background Cholesterol efflux from cells to apolipoprotein A‐I (apoA‐I) acceptors via the ATP‐binding cassette transporters ABCA1 and ABCG1 is thought to be central in the antiatherogenic mechanism. MicroRNA (miR)‐33 is known to target ABCA1 and ABCG1 in vivo. Methods and Results We assessed the impact of the genetic loss of miR‐33 in a mouse model of atherosclerosis. MiR‐33 and apoE double‐knockout mice (miR‐33−/−Apoe−/−) showed an increase in circulating HDL‐C levels with enhanced cholesterol efflux capacity compared with miR‐33+/+Apoe−/− mice. Peritoneal macrophages from miR‐33−/−Apoe−/− mice showed enhanced cholesterol efflux to apoA‐I and HDL‐C compared with miR‐33+/+Apoe−/− macrophages. Consistent with these results, miR‐33−/−Apoe−/− mice showed reductions in plaque size and lipid content. To elucidate the roles of miR‐33 in blood cells, bone marrow transplantation was performed in these mice. Mice transplanted with miR‐33−/−Apoe−/− bone marrow showed a significant reduction in lipid content in atherosclerotic plaque compared with mice transplanted with miR‐33+/+Apoe−/− bone marrow, without an elevation of HDL‐C. Some of the validated targets of miR‐33 such as RIP140 (NRIP1) and CROT were upregulated in miR‐33−/−Apoe−/− mice compared with miR‐33+/+Apoe−/− mice, whereas CPT1a and AMPKα were not. Conclusions These data demonstrate that miR‐33 deficiency serves to raise HDL‐C, increase cholesterol efflux from macrophages via ABCA1 and ABCG1, and prevent the progression of atherosclerosis. Many genes are altered in miR‐33‐deficient mice, and detailed experiments are required to establish miR‐33 targeting therapy in humans.
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Vir: Osebne bibliografije
in: SICRIS
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