This work proposes an automated strategy for the inorganic selenium speciation in infusion tea samples, employing an MSFIA-CVG-AFS system and sodium tetrahydroborate for chemical vapor generation. The selenium total is determined after an online prereduction step of selenium (VI) to selenium (IV) in alkaline media, using a UV reactor with a 15 W Hg lamp. Selenium (IV) is quantified directly on the sample, and selenium (VI) is determined by the difference between the total selenium and selenium (IV) concentrations. The optimization of the chemical parameters: hydrochloric acid – hydrobromide acid solution concentration, potassium iodide concentration, sodium hydroxide concentration, and sodium tetrahydroborate concentration was performed using a (24−1) two-level fractional factorial design. The validation parameters were determined for total selenium and selenium (IV), and the results found were: limits of detection and quantification of 0.05 and 0.18 μg L−1, respectively; a linear range of 0.18–5.0 μg L−1, precision expressed as the relative standard deviation of 2.1% for a sample number of 10, for both analytes. The system allows the speciation analysis with an injection throughput of 15 injections per hour. This analytical method was applied for inorganic selenium speciation in nine infusions of tea samples purchased commercially in supermarkets in Palma de Mallorca City, Spain. The concentrations of selenium (IV) and total selenium varied from 0.2 to 0.6 μg L−1 and 0.4–2.0 μg L−1, respectively. The accuracy method was evaluated using spike tests, and the recoveries achieved varied from 91 to 111%. Display omitted •A new system is proposed for inorganic selenium speciation in infusion tea samples using atomic fluorescence spectrometry.•This method do not requires any sample pre-treatment.•Selenium (IV) is quantified directly on the sample.•Total selenium is determined after an online pre-reduction to selenium (IV) using a UV reactor.•Selenium (VI) is determined by the difference between the total selenium and selenium (IV) concentrations.