U radu je opisan antibiogram bakterije Vibrio alginolyticus izdvojene iz farmski uzgajane atlantske bijele kozice (Litopenaeus vannamei) i njezina otpornost na teške metale. Kao uzročnik vibrioze V. alginolyticus može dovesti do propadanja cijele farme. Stoga je poduzeto istraživanje s ciljem da se pronađe djelotvoran antibiotik protiv bakterije V. alginolyticus te da se ustanovi razina njezine podnošljivosti na teške metale kako bi se ustanovila razina izloženosti uzgajanih kozica tim metalima. Istražena je osjetljivost na 14 antimikrobnih tvari: oksolinsku kiselinu (2 μg), ampicilin (10 μg), eritromicin (15 μg), furazolidon (15 μg), linkomicin (15 μg), kolistin sulfat (25 μg), oleandomicin (15 μg), doksiciklin (30 μg), fosfomicin (50 μg), florfenikol (30 μg), flumekvin (30 μg), tetraciklin (30 μg) i spiramicin (100 μg), te na četiri teška metala: živu (Hg2+), kadmij (Cd2+), krom (Cr6+) i bakar (Cu2+). Najučinkovitijom se pokazala oksolinska kiselina na koju je bilo osjetljivo 92,8% izolata V. alginolyticus. Na furazolidon je bilo osjetljivo 85,6%, na nitrofuratoin 79,8%, na tetracillin 78,8%, na doksiciklin 72,2% i na florfenikol 71,2% izolata. S druge strane, svi su izolati bili otporni na linkomicin. U testu podnošljivosti na teške metale svi su bakterijski izolati bili otporni na ione žive, kadmija i kroma, dok je samo 26,7% bilo otporno na ione bakra. This paper describes the antibiogram and heavy metal resistancepattern of Vibrio alginolyticus isolated from farmed white leg shrimp (Litopenaeus vannamei). V. alginolyticus has been recognized as a causative agent of vibriosis in shrimp culture and can devastate an entire shrimp farm. Therefore, this study was carried out to investigate the suitability of using antibiotics against V. alginolyticus, as well as its tolerance to heavy metals, to reveal the exposure level of cultured shrimp to heavy metals. In the present study, a total of 14 antibiotics; oxolinic acid (2 μg), ampicillin (10 μg), erythromycin (15 μg), furazolidone (15 μg), lincomycin (15 μg), colistin sulphate (25 μg), oleandomycin (15 μg), doxycycline (30 μg), fosfomycin (50 μg), florfenicol (30 μg), flumequine (30 μg), tetracycline (30 μg), fosfomycin (50 μg) and spiramycin (100 μg) as well as four heavy metals; mercury (Hg2+), cadmium (Cd2+), chromium (Cr6+) and copper (Cu2+) were applied. Based on the results for antibiotic sensitivity, it was shown that oxolinic acid is the most effective antibiotic in controlling V. alginolyticus, where 92.8% of the bacterial isolates present were sensitive to it. It was followed by furazolidone (85.6%), nitrofuratoin (79.8%), tetracycline (78.8%), doxycycline (72.2%) and florfenicol (71.2%). On the other hand, all the bacterial isolates present were resistant to lincomycin. In the heavy metal tolerance test, all the bacterial isolates present were resistant to Hg2+, Cd2+ and Cr6+ whereas only 26.7% of them were resistant to Cu2+.