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Jeynes, J. Charles. G; Jeynes, Christopher; Merchant, Michael J; Kirkby, Karen J
10/2013, Letnik: 138, Številka: 23Journal Article
The cell-to-cell variation of gold nanoparticle (GNP) uptake is important for therapeutic applications. We directly counted the GNPs in hundreds of individual cells, and showed that the large variation from cell-to-cell could be directly modelled by assuming log-normal distributions of both cell mass and GNP rate of uptake. This was true for GNPs non-specifically bound to fetal bovine serum or conjugated to a cell penetrating peptide. Within a population of cells, GNP content varied naturally by a factor greater than 10 between individual cells. Microbeam proton beam analysis is used to measure the total carbon and gold content of individual cancer cells to determine cell-to-cell variability in gold nanoparticle uptake.
