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Kocijan, Andrej; Grahek, Rok; Bastarda, Andrej; Kralj, Lucija Zupančič
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 08/2005, Letnik: 822, Številka: 1Journal Article
High throughput methods (high performance liquid chromatography and capillary electrophoresis) were developed to determine pravastatin in production media. The analyses were performed on particle column, monolithic column and silica capillary filled with borate buffer pH 9.3 containing 20 mM SDS. All three methods successfully separate pravastatin from interfering compounds (matrix, mevastatin and 6- epi pravastatin) and runtimes are shorter than 1 min. Solvent consumptions for methods using small particle column, monolith column and MECK were 132, 510 and 1.5 mL h −1. The most sensitive was the method using particle column (LOD was about 10 −5 mg mL −1), followed by the system using monolith column (LOD was 2 × 10 −4 mg mL −1) and the MECK method (LOD was about 0.02 mg mL −1).
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