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Andrianova, M. S.; Gubanova, O. V.; Komarova, N. V.; Kuznetsov, E. V.; Kuznetsov, A. E.
Electroanalysis (New York, N.Y.), June 2016, Letnik: 28, Številka: 6Journal Article
A biosensor for the direct determination of organophosphorus pesticides (OPs) is described. It utilizes an ion‐selective field‐effect transistor and phosphotriesterase enzyme (PTE), which provides cleavage of OPs. The sensitivity of the biosensor was increased after PTE immobilization on the surface of the transistor. Packaging of the integrated circuit and creation of a microfluidic system for analyte delivery stabilized the signal. This system was able to detect 0.1 µM paraoxon and 0.5 µM parathion and methyl parathion and was stable for at least 1 month (PBS, 1°), and could thus provide the basis for a portable device for analyzing OPs in water.
