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Wu, Yan; Jiang, Tingting; Wu, Zhaoyang; Yu, Ruqin
Talanta (Oxford), 08/2018, Letnik: 185Journal Article
A ratiometric surface-enhanced Raman scattering (SERS) aptasensor based on internal standard (IS) methods was proposed for the ultrasensitive and reproducible quantitative detection of silver ion (Ag+) with Au@Ag core-shell nanoparticle (Au@Ag NP) substrate. In principle, the thiolated 5′-Rox C-containing labeled aptamer probe (Rox-aptamer) is firstly immobilized on the SERS substrate surface and then hybridizes with the complementary DNA (cDNA) to form a rigid double-stranded DNA (dsDNA), in which the Rox Raman labels is used to produce the Raman signal. Furthermore, the pyridine is employed as an IS element to provide the ratiometric determination of target. In the presence of Ag+, the Rox-aptamer is turned into the cytosine (C)-Ag+-C mediated hairpin structure, which remarkably reduces the distance between the Rox labels and the Au@Ag NP surface responsible for a measurable ‘turn-on’ signal change of Rox. This IS-based ratiometric SERS aptasensor exhibits a limit of detection of 50 pM for Ag+ with a linear detection range from 0.1 to 100 nM and the shortcoming of irreproducibility of SERS signal could be overcome. The proposed method provides a simple, robust, and rapid approach for the sensitive and reproducible quantitative detection of Ag+, and it could also be used for the detection of other metal ions which exhibits specific interactions with natural or synthetic bases. Display omitted •An internal standard-based ratiometric SERS aptasensor was developed for ultrasensitive quantitative Ag+ detection.•The ratiometric SERS aptasensor achieves a limit of detection (LOD) to 50 pM Ag+.•This strategy may offer a new direction for development of high performance SERS aptasensors for other metal ions detection.
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