The presence of bioactive compounds in winery waste streams represents a great opportunity to revalue these effluents that constitute a challenging environmental and economic problem. The main objective of this research is to develop an efficient and environmentally friendly liquid-liquid extraction process for the recovery of phenolic antioxidants from winery wastewater. Two bio-based solvents, 2-methyltetrahydrofuran (2-MeTHF) and cyclopentyl methyl ether (CPME), were evaluated as potential extractants along with ethyl acetate (EA) as benchmark organic solvent. Extraction efficiencies were maximized by testing several operation factors such as stirring time, solvent to feed ratio, concentration and alcohol effect. Subsequently, the extracted phenolic compounds were quantified by spectrophotometric (using the Folin-Ciocalteu assay, the flavonoid-aluminum chloride complexation assay and the DPPH free radical assay) and chromatographic (HPLC-DAD) methods. Overall, 2-MeTHF showed the highest extraction efficiency of target antioxidants, yielding up to 96.46% for gallic acid, 87.44% for protocatechuic acid, 80.82% for 4-hydroxybenzoic acid, 92.24% for caffeic acid, 75.18% for vanillic acid and 38.19% for syringic acid. In the interest of full sustainability, the isolation of the phenolic compounds as a solid fraction from the extraction solvent was efficiently accomplished, while the recyclability of 2-MeTHF was successfully demonstrated. Display omitted •Sustainable recovery of antioxidants was proposed for winery wastewater valorization.•Bio-based solvents extracted target phenolic antioxidants from winery effluents.•2-MeTHF showed the highest extraction yield over the conventional solvent.•LLE optimization was performed using spectrophotometric/chromatographic methods.•2-MeTHF was efficiently recycled and reused in the extraction process.