In addition to their active ingredients, pesticides contain also additives – surfactants. Use of surfactants has been increasing over the past decade, but their effects on non-target organisms, especially natural enemies of pests, have been studied only very rarely. The effect of three common agrochemical surfactants on the foraging behavior of the wolf spider Pardosa agrestis was studied in the laboratory. Differences in short-term, long-term, and overall cumulative predatory activities were investigated. We found that surfactant treatment significantly affected short-term predatory activity but had no effect on long-term predatory activity. The surfactants also significantly influenced the cumulative number of killed prey. We also found the sex-specific increase in cumulative kills after surfactants treatment. This is the first study showing that pesticide additives have a sublethal effect that can weaken the predatory activity of a potential biological control agent. More studies on the effects of surfactants are needed to understand how they affect beneficial organisms in agroecosystems. Display omitted •Foraging behavior of Pardosa agrestis was studied after agronomical surfactant treatments.•Short- and long-term predatory activity after surfactant treatment was recorded.•Agronomical surfactants can weaken predatory activity of potential biocontrol agent. Agronomical surfactants have sublethal effect on Pardosa agrestis. They influence the prey capture rate and weaken the predatory activity of a potential biological control agent.