In this paper, numerical and experimental modal analysis of a segmented wind turbine blade assembled with a steel threaded shaft and a nut are presented. The blade segments are built by a 3D printer using ABS material. The experimental modal parameters identification has been achieved using the Eigen system Realization Algorithm (ERA) method for different values of the blade segments assembly force caused by the nut tightening torque. Furthermore, a three dimensional finite element model has been built using DTK18 three node triangular shell elements in order to model the blade and the threaded shaft structure, taking into account the additional stiffness caused by the nut tightening torque. This study covers the blade segments assembly force effects on the rotating blade vibration characteristics. The numerical model is adjusted and validated by the identified experimental results. This work highlights the significant variation of the natural frequencies of the segmented wind turbine blade by the assembling load of the segments versus blade rotating speed.