A glasshouse pot experiment was conducted to investigate the effect of two species of arbuscular mycorrhizal fungi (Glomus intraradices and Acaulospora laevis) and Pseudomonas fluorescens under three levels of superphosphate fertilizer (half dose, 40 kg/ha; recommended dose, 80 kg/ha; and double dose, 160 g/ha) on the growth and yield of broccoli (Brassica oleracea var. italica). There was negligible colonization of broccoli roots by the mycorrhizal fungi, demonstrating that they have no role in the growth of this plant. However, the strain of P. fluorescens used (MTCC No. 103) was found to have a potential role in enhancing the growth, phosphatase activity, chlorophyll content, nutrient uptake and yield of broccoli when combined with the recommended dose of fertilizer. By contrast, P. fluorescens combined with a half or double dose of fertilizer led to poor growth and delayed head formation. This suggests that root inoculation with P. fluorescens (MTCC No. 103) will improve plant growth, nutrient uptake and yield of broccoli when combined with the recommended dose of superphosphate fertilizer.