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IANNELLI, F; GIUNTI, L; POZZI, G
Molecular biotechnology, 10/1998, Letnik: 10, Številka: 2Journal Article
Direct sequencing of polymerase chain reaction (PCR)-generated templates is a commonly used technique in molecular biology laboratories. We describe an improved method for direct sequencing of PCR fragments longer than 20 kb obtained with a commercial mixture of Taq and Pwo DNA polymerases. The sequencing protocol was optimized for an automated infrared DNA sequencer, consistently yielding long reads (500-600 bases).
