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Liu, Wei-Min; Skinner, Simon P.; Timmer, Monika; Blok, Anneloes; Hass, Mathias A. S.; Filippov, Dmitri V.; Overhand, Mark; Ubbink, Marcellus
Chemistry : a European journal, May 19, 2014, Letnik: 20, Številka: 21Journal Article
Paramagnetic NMR probes provide valuable long‐range structural information on proteins and protein complexes. A new, stable, two‐armed lanthanoid probe is reported that can be attached to a protein site‐specifically via chemically inert thioether linkages. A two‐armed probe: α‐Bromoacetamides are functionalised in a DOTA‐like structure to form a new double‐arm paramagnetic probe (see structure). This probe can be site‐specifically attached to proteins and the ligation products are stable under reducing conditions. This probe creates a new avenue for studying biomolecules using paramagnetic NMR spectroscopy.
