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Brown, Ronald D.; Armentrout, R. W.; Cochran, Mark D.; Cappello, Joe; Langemeier, Suzanne O.
Proceedings of the National Academy of Sciences - PNAS, 03/1981, Letnik: 78, Številka: 3Journal Article
A recombinant cDNA plasmid containing Xenopus immunoglobulin heavy chain sequence has been constructed from Xenopus spleen poly(A)-containing RNA. The plasmid was identified by colony hybridization and a hybridization-translation assay and its identity was confirmed by DNA sequence analysis. The portion of the heavy chain sequence contained in the plasmid is 35% homologous to mammalian μ and γ sequences. The mRNA corresponding to this plasmid is 2.5 kilobases, in close agreement with the size of mouse μ mRNA. RNA sequences complementary to the cloned sequence appear in embryos about 24 hr after fertilization, which corresponds to 24 hr before the first detectable immunoglobulin.
