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Leijon, Mikael; Graeslund, Astrid; Nielsen, Peter E; Buchardt, Ole; Norden, Bengt; Kristensen, Soren M; Eriksson, Magdalena
Biochemistry (Easton), 08/1994, Letnik: 33, Številka: 33Journal Article
The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by 1H NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.
