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Cherrier, Mickaël V; Vernède, Xavier; Fenel, Daphna; Martin, Lydie; Arragain, Benoit; Neumann, Emmanuelle; Fontecilla-Camps, Juan C; Schoehn, Guy; Nicolet, Yvain
Biomolecules (Basel, Switzerland), 03/2022, Letnik: 12, Številka: 3Journal Article
Metalloproteins are involved in key cell processes such as photosynthesis, respiration, and oxygen transport. However, the presence of transition metals (notably iron as a component of Fe-S clusters) often makes these proteins sensitive to oxygen-induced degradation. Consequently, their study usually requires strict anaerobic conditions. Although X-ray crystallography has been the method of choice for solving macromolecular structures for many years, recently electron microscopy has also become an increasingly powerful structure-solving technique. We have used our previous experience with cryo-crystallography to develop a method to prepare cryo-EM grids in an anaerobic chamber and have applied it to solve the structures of apoferritin and the 3 Fe S -containing pyruvate ferredoxin oxidoreductase (PFOR) at 2.40 Å and 2.90 Å resolution, respectively. The maps are of similar quality to the ones obtained under air, thereby validating our method as an improvement in the structural investigation of oxygen-sensitive metalloproteins by cryo-EM.
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