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Andrés-Benito, Pol; Flores, África; Busquet-Areny, Sara; Carmona, Margarita; Ausín, Karina; Cartas-Cejudo, Paz; Lachén-Montes, Mercedes; Del Rio, José Antonio; Fernández-Irigoyen, Joaquín; Santamaría, Enrique; Ferrer, Isidro
International journal of molecular sciences, 03/2023, Letnik: 24, Številka: 7Journal Article
Transcriptomics and phosphoproteomics were carried out in the cerebral cortex of (tau knockout: tau-KO) and wild-type (WT) 12 month-old mice to learn about the effects of tau ablation. Compared with WT mice, tau-KO mice displayed reduced anxiety-like behavior and lower fear expression induced by aversive conditioning, whereas recognition memory remained unaltered. Cortical transcriptomic analysis revealed 69 downregulated and 105 upregulated genes in tau-KO mice, corresponding to synaptic structures, neuron cytoskeleton and transport, and extracellular matrix components. RT-qPCR validated increased mRNA levels of , , , , , , , , , and an absence of in tau-KO mice compared with WT mice. A few proteins were assessed with Western blotting to compare mRNA expression with corresponding protein levels. mRNA and protein levels decreased. However, β-tubulin III and GAD1 protein levels were reduced in tau-KO mice. Cortical phosphoproteomics revealed 121 hypophosphorylated and 98 hyperphosphorylated proteins in tau-KO mice. Deregulated phosphoproteins were categorized into cytoskeletal ( = 45) and membrane proteins, including proteins of the synapses and vesicles, myelin proteins, and proteins linked to membrane transport and ion channels ( = 84), proteins related to DNA and RNA metabolism ( = 36), proteins connected to the ubiquitin-proteasome system (UPS) ( = 7), proteins with kinase or phosphatase activity ( = 21), and 22 other proteins related to variegated pathways such as metabolic pathways, growth factors, or mitochondrial function or structure. The present observations reveal a complex altered brain transcriptome and phosphoproteome in tau-KO mice with only mild behavioral alterations.
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