The Northern Bald Ibis is an endangered species, and recently it was reintroduced in Europe by the Waldrappteam. The reintroduction program has been strongly threatened by several sudden deaths, mainly in Italy. The present study used a forensic approach to analyze all of the 27 Northern Bald Ibises found dead in Italy between 2016 and 2022, which were suspected to have been victims of poaching, and it followed the veterinary forensic guidelines. Human-related causes accounted for 60% of the deaths, including illegal hunting (30%), blunt force trauma (26%), and electrocution (4%). Natural causes, starvation (15%), predation (11%) and disease (7%), accounted for 33% of the deaths. 7% of the causes of deaths remained undetermined. This study uses a forensic approach to analyze, for the first time, the main causes of death in and highlights the relevance of detecting illegal actions related to endangered species and stopping the phenomenon of poaching.