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  • Towards a whole‐genome sequ...
    Bauer, Eva; Schmutzer, Thomas; Barilar, Ivan; Mascher, Martin; Gundlach, Heidrun; Martis, Mihaela M.; Twardziok, Sven O.; Hackauf, Bernd; Gordillo, Andres; Wilde, Peer; Schmidt, Malthe; Korzun, Viktor; Mayer, Klaus F.X.; Schmid, Karl; Schön, Chris‐Carolin; Scholz, Uwe

    The Plant journal : for cell and molecular biology, March 2017, Letnik: 89, Številka: 5
    Journal Article

    Summary We report on a whole‐genome draft sequence of rye (Secale cereale L.). Rye is a diploid Triticeae species closely related to wheat and barley, and an important crop for food and feed in Central and Eastern Europe. Through whole‐genome shotgun sequencing of the 7.9‐Gbp genome of the winter rye inbred line Lo7 we obtained a de novo assembly represented by 1.29 million scaffolds covering a total length of 2.8 Gbp. Our reference sequence represents nearly the entire low‐copy portion of the rye genome. This genome assembly was used to predict 27 784 rye gene models based on homology to sequenced grass genomes. Through resequencing of 10 rye inbred lines and one accession of the wild relative S. vavilovii, we discovered more than 90 million single nucleotide variants and short insertions/deletions in the rye genome. From these variants, we developed the high‐density Rye600k genotyping array with 600 843 markers, which enabled anchoring the sequence contigs along a high‐density genetic map and establishing a synteny‐based virtual gene order. Genotyping data were used to characterize the diversity of rye breeding pools and genetic resources, and to obtain a genome‐wide map of selection signals differentiating the divergent gene pools. This rye whole‐genome sequence closes a gap in Triticeae genome research, and will be highly valuable for comparative genomics, functional studies and genome‐based breeding in rye. Significance statement Rye is a diploid Triticeae species closely related to wheat and barley. Here we present a draft genome and a high‐density genotyping array that will facilitate genome‐based research in rye and comparative genomics in Triticeae.