Abstract Currency is one of the most frequently passed items in the world. It used every day and every way, and it could act as an important vehicle for cross-contamination. This research was aimed to determine how long pathogenic bacteria can survive on currency paper, four pathogenic bacteria including E. coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis were provided according to laboratory standard methods. Sterile pieces of currency paper were inoculated with the pathogenic bacteria and the survival of bacteria was tested over seven days. The results showed that all tested bacteria differed in time of survival and this survives according to environmental conditions, the type of bacteria and the mode of its resistance, but they were stable and still cultivable after seven days. The second part of this research was to design a simple experimental model to check the efficacy of germicidal and attempted to use ultraviolet light radiation to evaluate the inhibitory effect of most pathogenic microorganisms on currency as a disinfectant. The results revealed that UV is an effective tool, so we recommend using it as a disinfectant for currency.