We present refined cosmological parameter constraints derived from a cosmic shear analysis of the fourth data release of the Kilo-Degree Survey (KiDS-1000). Our main improvements include enhanced galaxy shape measurements made possible by an updated version of the lens fit code and improved shear calibration achieved with a newly developed suite of multi-band image simulations. Additionally, we incorporated recent advancements in cosmological inference from the joint Dark Energy Survey Year 3 and KiDS-1000 cosmic shear analysis. Assuming a spatially flat standard cosmological model, we constrain S 8  ≡  σ 8 (Ω m /0.3) 0.5  = 0.776 −0.027−0.003 +0.029+0.002 , where the second set of uncertainties accounts for the systematic uncertainties within the shear calibration. These systematic uncertainties stem from minor deviations from realism in the image simulations and the sensitivity of the shear measurement algorithm to the morphology of the galaxy sample. Despite these changes, our results align with previous KiDS studies and other weak lensing surveys, and we find a ∼2.3 σ level of tension with the Planck cosmic microwave background constraints on S ​ 8 .