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Virant, David; Traenkle, Bjoern; Maier, Julia; Kaiser, Philipp D; Bodenhöfer, Mona; Schmees, Christian; Vojnovic, Ilijana; Pisak-Lukáts, Borbála; Endesfelder, Ulrike; Rothbauer, Ulrich
Nature communications, 03/2018, Letnik: 9, Številka: 1Journal Article
Dense fluorophore labeling without compromising the biological target is crucial for genuine super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for fixed and living cells utilizing a short peptide tag-specific nanobody (BC2-tag/bivBC2-Nb). BC2-tagging of ectopically introduced or endogenous proteins does not interfere with the examined structures and bivBC2-Nb staining results in a close-grained fluorophore labeling with minimal linkage errors. This allowed us to perform high-quality dSTORM imaging of various targets in mammalian and yeast cells. We expect that this versatile strategy will render many more demanding cellular targets amenable to dSTORM imaging.
