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Kędrak-Jabłońska, Agnieszka; Budniak, Sylwia; Szczawińska, Anna; Reksa, Monika; Krupa, Marek; Szulowski, Krzysztof
Journal of veterinary research, 12/2018, Letnik: 62, Številka: 4Journal Article
The aim of the study was the application and evaluation of real-time PCRs based on the fluorescence of SYBR Green I intercalating dye for the detection of three genes in contaminated liver and blood samples. The goals for detection were gene as a chromosomal marker, gene located on plasmid pXO1, and gene located on plasmid pXO2. Five strains were used for the experiments. Additionally, single strains of other species of the genus , . , , , and , and strains of six other species were used for evaluation of the specificity of the tests. Three SYBR Green I real-time PCRs were conducted allowing confirmation of in the biological samples. The observation of amplification curves in real-time PCRs enabled the detection of the chromosomally encoded gene, gene, and gene of . The specificity of the tests was confirmed by estimation of the melting temperature of the PCR products. The sensitivity and linearity of the reactions were determined using regression coefficients. Strains of other microbial species did not reveal real-time PCR products. All real-time PCRs for the detection of in biological samples demonstrated a significant sensitivity and high specificity.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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