E-viri
Recenzirano
Odprti dostop
-
McEwan, David G.; Richter, Benjamin; Claudi, Beatrice; Wigge, Christoph; Wild, Philipp; Farhan, Hesso; McGourty, Kieran; Coxon, Fraser P.; Franz-Wachtel, Mirita; Perdu, Bram; Akutsu, Masato; Habermann, Anja; Kirchof, Anja; Helfrich, Miep H.; Odgren, Paul R.; Van Hul, Wim; Frangakis, Achilleas S.; Rajalingam, Krishnaraj; Macek, Boris; Holden, David W.; Bumann, Dirk; Dikic, Ivan
Cell host & microbe, 01/2015, Letnik: 17, Številka: 1Journal Article
The host endolysosomal compartment is often manipulated by intracellular bacterial pathogens. Salmonella (Salmonella enterica serovar Typhimurium) secrete numerous effector proteins, including SifA, through a specialized type III secretion system to hijack the host endosomal system and generate the Salmonella-containing vacuole (SCV). To form this replicative niche, Salmonella targets the Rab7 GTPase to recruit host membranes through largely unknown mechanisms. We show that Pleckstrin homology domain-containing protein family member 1 (PLEKHM1), a lysosomal adaptor, is targeted by Salmonella through direct interaction with SifA. By binding the PLEKHM1 PH2 domain, Salmonella utilize a complex containing PLEKHM1, Rab7, and the HOPS tethering complex to mobilize phagolysosomal membranes to the SCV. Depletion of PLEKHM1 causes a profound defect in SCV morphology with multiple bacteria accumulating in enlarged structures and significantly dampens Salmonella proliferation in multiple cell types and mice. Thus, PLEKHM1 provides a critical interface between pathogenic infection and the host endolysosomal system. Display omitted •PLEKHM1 loss impairs Salmonella proliferation and vacuole formation•PLEKHM1 simultaneously interacts with Salmonella effector SifA and Rab7 via a PH2 domain•SifA recruits the PLEKHM1/Rab7/HOPS complex for SCV maintenance Salmonella hijack the lysosomal compartment to provide a stable intracellular proliferative niche. McEwan et al. show that the Salmonella effector SifA recruits the HOPS complex and Rab7 through PLEKHM1. Loss of PLEKHM1 decreases Salmonella proliferation and alters vacuole structure, leading to multiple Salmonella encased in enlarged structures.
Avtor
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.