To determine whether there was an increased prevalence of low serum cobalamin concentrations in elderly people. Serum concentrations of cobalamin were measured in 202 elderly people. In the elderly group, cobalamin concentrations less than 191 pg/mL were found in 79 subjects (39.1%) compared with 24.5% of 19–40 years old subjects ( n = 527) and 29.7% of 41–64 years old subjects ( n = 1100) ( p < 0.001, p < 0.05, respectively). The serum cobalamin concentration in the 202 elderly group (262 ± 201 pg/mL) was not significantly different than that of the 19–40 years old subjects (280 ± 164 pg/mL) and 41–64 years old subjects (275 ± 152 pg/mL). Anemia was seen in 5% of elderly subjects with cobalamin values  < 191 pg/mL. Also macrocytosis was determined in 5 subjects (6.3%). The prevalence of cobalamin deficiency was 39.1% in Afyonkarahisar elderly population. Many elderly people with low serum cobalamin concentration had not had hematological findings.