To efficiently process complex visual scenes, the visual system often summarizes statistical information across individual items and represents them as an ensemble. However, due to the lack of techniques to disentangle the representation of the ensemble from that of the individual items constituting the ensemble, whether there exists a specialized neural mechanism for ensemble processing and how ensemble perception is computed in the brain remain unknown. To address these issues, we used a frequency-tagging EEG approach to track brain responses to periodically updated ensemble sizes. Neural responses tracking the ensemble size were detected in parieto-occipital electrodes, revealing a global and specialized neural mechanism of ensemble size perception. We then used the temporal response function to isolate neural responses to the individual sizes and their interactions. Notably, while the individual sizes and their local and global interactions were encoded in the EEG signals, only the global interaction contributed directly to the ensemble size perception. Finally, distributed attention to the global stimulus pattern enhanced the neural signature of the ensemble size, mainly by modulating the neural representation of the global interaction between all individual sizes. These findings advocate a specialized, global neural mechanism of ensemble size perception and suggest that global interaction between individual items contributes to ensemble perception.