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McCarty, K.J.; DeCarlo, A.N.; Ricks, R.E.; Pratt, S.L.; Long, N.M.
Animal reproduction science, April 2024, 2024-Apr, 2024-04-00, 20240401, Letnik: 263Journal Article
The objective was to determine the effects of maternal nutrient restriction during gestation on serum microRNA (miRNA) abundance in cattle. Primiparous Angus-cross cows (n=22) were fed either control (CON; to gain 1 Kg/week) or nutrient restricted (NR; 0.55% NEm) diets based on National Research Council requirements. On day 30 of gestation, cows were blocked by body condition and randomly assigned to one of three diets: CON (n=8) days 30–190; NR (n=7) days 30–110 followed by CON days 110–190 (NR/C); or CON (n=7) days 30–110 followed by NR days 110–190 (C/NR). At 190 days of gestation, maternal serum was collected for RNA isolation and analyzed using a miRNA microarray of known Bos taurus sequences. Data were normalized using LOWESS and analyzed via ANOVA. At 190 days of gestation, 16 miRNAs exhibited differential abundance (P<0.05) between treatments. Cows that underwent NR, irrespective of when the insult occurred, had downregulated bta-miR-126–3p compared to CON cows. Bta-miR-16b was downregulated and three miRNAs upregulated in NR/C compared to C/NR and CON cows. Additionally, seven miRNAs were downregulated and four miRNAs upregulated in C/NR compared to NR/C and CON cows. Comparison of NR/C and C/NR cows revealed three differentially abundant (P<0.04) miRNAs (bta-miR-2487_L-2R-3_1ss15CT, bta-miR-215, and bta-miR-760–5p). Top KEGG pathway enrichment of target genes included: pathways in cancer, PI3K-Akt signaling, focal adhesion, Ras signaling, proteoglycans in cancer, and MAPK signaling. In summary, maternal nutrient restriction altered serum miRNA abundance profiles irrespective of the time at which the nutritional insult was induced. •Nutrient restriction altered serum miRNA abundance irrespective of time of insult.•Maternal serum at 190 days of gestation have 16 microRNAs of differential abundance.•The 16 miRNAs of interest are predicted to target a total of 7,576 genes.•Pathway analysis revealed a total of 106 enriched KEGG pathways.•Top three pathways: pathways in cancer, PI3K-Akt signaling, and focal adhesion.
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Vir: Osebne bibliografije
in: SICRIS
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