Fungal decomposition of wood severely affects the soundness of timber constructions. The diagnosis of wood decay requires direct observations or sampling by skilled experts. Wood decay often occurs in obscure spaces, including the enclosed inner spaces of walls or under the floor. In this study, we examined the ability of machine olfaction to detect odors of fungi grown on common construction softwoods to provide a novel diagnostic method for wood construction soundness. The combination of a simple device equipped with semiconductor gas sensors (gas sensor array) and multivariate analysis discriminated a fungi-related odor from control odor without instrumental analysis (e.g., gas chromatography). This method is often referred to as machine olfaction or electronic nose. We measured the odor of wood test pieces that were infected with Fomitopsis palustris or Trametes versicolor and sound test pieces using a gas sensor array. The sensor responses of the specimens showed different patterns between the inoculated and control samples. Each specimen class formed independent groups in a principal component score plot, almost regardless of wood species, fungal species, or cultivation period. This method provides a new decay diagnosis method that is cost-effective and easy to operate.